Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences HEPES anhydrous free flowi25KG
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences HEPES sodium salt BioPerfo1KG
HEPES sodium salt BioPerfo1KG
Quality Biological Inc PBS pH 7.4 without Calcium and Magnesium, CS 10 X 500 mL
PBS (phosphate buffered saline) ¿ Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. ¿ Endotoxin and Mycoplasma tested ¿ Applications: immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ pH: 7.3 ¿ 7.5 ¿ Osmolality: 300¿5% mOsm/Kg H2O ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Quality Biological Inc TBS 10X pH 7.4, CS 10 X 1000 mL
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc Tris HCl (1 M), pH 7.5, 1000 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences ADA 98 titration1KG
ADA is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960 to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous SOLUTIONon is 6.0 - 7.2.
Sigma Aldrich Fine Chemicals Biosciences DIPSO BioXtra pH 4.0 5.5 2100G
DIPSO BioXtra pH 4.0 5.5 2100G
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
Quality Biological Inc PBS (10X), pH 7.4, 500 mL
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ Max Shelf Life: 24 months
Quality Biological Inc Tris HCL (1 M), pH 8.0, 500 mL
A hybridization buffer specifically formulated for use with nylon membranes. ¿ It does not contain Denhardt¿s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. ¿ Formulated for Nylon membrane ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Denatured fragmented salmon sperm DNA may be added to this hybridization buffer to reduce nonspecific background. ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML
BioMed Diagnostics An isotonic solution typically used for detection of the severe acute respiratory syndrome SARS-CoV-2. | Available in: 50 pack
This Saline Solution (0.85%) has been validated for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19 disease in humans. | Product Principle: 1.) Intended to aid in the process of collection and transport of human clinical specimen. 2.) Ensures a non-replicating competent status of SARS-CoV-2. 3.) Is aseptic and does not contain bacteria or fungi growth-inhibition agents. 4.) Validated to be compatible with the identification of SARS-CoV-2 using qRT-PCR technology | Detects: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). | Available in: 50 pack
Promocell GmbH PromoCell Hepes BSS (30 mM) 30 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).
Promocell GmbH PromoCell Hepes BSS (30 mM) 250 ml, HEPES Buffered Saline Solution
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.HEPES Buffered Saline Solution (30 mM).