Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 25 g
MES monohydrate | Purity: ≥99.0% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 25 g
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 | C8H18N2O4S·1/2Na | 100 MG
HEPES hemisodium salt is a zwitterionic buffering reagent commonly used to maintain physiological pH in biochemical assays and cell-culture media. Supplied as a solid powder, it is intended for preparation of HEPES buffer solutions and includes storage and handling guidance for laboratory use.
- High buffering capacity at physiological pH (6.8-8.2).
- Available as a solid powder for convenient solution preparation.
- Reported high purity suitable for analytical and cell-culture applications.
- Stable with sealed storage at 4°C and protected from moisture.
- Provided with supporting documentation (datasheet and safety information).
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Research Products International Corp PIPES Sodium Salt 500 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp MES Sodium Salt 5 KG
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.;
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
Boston Bioproducts Inc TAPS Buffer (1 M, pH 8.5) - 100ml
TAPS Buffer (1 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC HEPES, NaCl, Sucrose, pH 7.2 buffer | 100 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is primarily used to resuspend human erythrocyte ghost preparations.
- Physiological buffer
- Composed of HEPES, NaCl, and Sucrose
- Adjusted to pH 7.2
- Used to resuspend human erythrocyte ghost preparations
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Aniara Diagnostica LLC PBS TBLT PH 7.4 1000ML 100 EA
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
Electron Microscopy Sciences Phosphate Buffered Saline, 2X 1 GL
PBS is commonly used in biochemistry. It is a salty solution containing sodium chloride, potassium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 8.0 g/L NaCl, 0.2 g/L KCl, 1.42 g/L Na2HPO4, 0.24 g/L KH2PO4 and pH 7.4.
Cell Signaling Technology Tris Buffered Saline (TBS-10X) 1 liter
Tris Buffered Saline (TBS-10X) 1 liter
Quality Biological Inc Tris (1 M) pH 9.8 1000mL
Suitable for molecular biology protein chemistry and biochemical applications.DNase RNase and Protease tested.Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.